Amine Oxidases: Crystallization and Characterization
نویسندگان
چکیده
منابع مشابه
Amine Oxidases of Microorganisms Part II. Purification and Crystallization of Amine Oxidase of Aspergillus niger
A procedure for obtaining crystalline preparations of the amine oxidase of Aspergillus niger has been developed. The method involved fractionations with ammonium sulfate and separation on successive columns of DEAE-cellulose, DEAE-sephadex and hydroxyl apatite. Crystals were formed when solid ammonium sulfate was added to solutions of the purified enzyme (of about 300to 350-times the specific a...
متن کاملSubstrate specificity of amine oxidases.
DuBois, K. P., Albaum, H. G. & Potter, V. R. (1943). J. biol. Chem. 147, 699. Green, H. N. (1943). Lancet, 2, 147. Green, H. N. & Stoner, H. B. (1944). Brit. J. exp. Path. 25, 150. Kal¢kar, H. M. (1943). J. biol. Chem. 148, 127. Kolthoff, I. M. (1932). Saure-Ba8en Indicatoren. Berlin: Springer. Krebs, H. A. & Henseleit, K. (1932). Ho.ppe-Seyl. Z. 210, 33.' Lyubimova, M. N. & Pevsner, D. (1941)....
متن کاملInhibition of chickpea seedling copper amine oxidases by tetraethylenepentamine
Copper amine oxidases are important enzymes, which contribute to the regulation of mono- and polyamine levels. Each monomer contains one Cu(II) ion and 2,4,5-trihydroxyphenylalanine (TPQ) as cofactors. They catalyze the oxidative deamination of primary amines to aldehydes with a ping-pong mechanism consisting of a transamination. The mechanism is followed by the transfer of two electrons to mol...
متن کاملCloning and characterization of Arabidopsis and Brassica juncea flavin-containing amine oxidases.
Polyamines (PAs) are low molecular weight metabolites involved in various physiological and developmental processes in eukaryotic and prokaryotic cells. The cellular PA level is regulated in part by the action of amine oxidases (AOs) including copper diamine oxidases (DAOs) and flavoprotein polyamine oxidases (PAOs). In this study, the isolation and characterization of flavin amine oxidases (FA...
متن کاملThe amine oxidases of human placenta and pregnancy plasma.
1. The purification of monoamine oxidase and diamine oxidase from normal human term placental tissue is described. 2. The properties of these enzymes are reported and compared with the properties of unpurified human pregnancy plasma. 3. This comparison shows that the amine oxidase of pregnancy plasma has properties corresponding to purified placental diamine oxidase, suggesting a placental orig...
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ژورنال
عنوان ژورنال: Journal of the agricultural chemical society of Japan
سال: 1967
ISSN: 0002-1407,1883-6844
DOI: 10.1271/nogeikagaku1924.41.3_r15